cutsmart buffer  (New England Biolabs)

Journal: bioRxiv

Article Title: MacroH2A-Mediated Gene Repression through Nucleosome Compaction and Remodeling Inhibition

doi: 10.64898/2026.01.26.701634

Figure Lengend Snippet: A. Representation of the protein domains, used in this study. B. The 601 Widom DNA with the two restriction-enzyme cutting site indicated. C. Representative acrylamide gels of the endonuclease assay of canonical and different truncated mH2A nucleosomes. D. Quantification of the digestion shown in (C). The left panel is the HinfI digestion result and the right is BtsCI digestion results. Data are mean ± SD, n = 3. Statistical significance was indicated by asterisk.

Article Snippet: 250 nM of the 167-bp nucleosomes were mixed with either 45 U of the HinfI endonuclease or 4.5 U of the BtsCI endonuclease in Cutsmart buffer (NEB) [20 mM Tris-Ac (pH 7.9), 50 mM KAc, 10 mM MgAc, and 100 μg/mL BSA].

Techniques:

Journal: bioRxiv

Article Title: MacroH2A-Mediated Gene Repression through Nucleosome Compaction and Remodeling Inhibition

doi: 10.64898/2026.01.26.701634

Figure Lengend Snippet: (A) Representative acrylamide gels show HinfI digestion (left) and BtsCI digestion (right) of H2A and various mH2A nucleosomes. (B) Quantification of the results in (A). The left panel is the HinfI digestion result and the right is BtsCI digestion results. Data are mean + SD, n=3.

Article Snippet: 250 nM of the 167-bp nucleosomes were mixed with either 45 U of the HinfI endonuclease or 4.5 U of the BtsCI endonuclease in Cutsmart buffer (NEB) [20 mM Tris-Ac (pH 7.9), 50 mM KAc, 10 mM MgAc, and 100 μg/mL BSA].

Techniques: